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MagPure Circulating DNA Rich Maxi Kit

5 mL Representative Plasma Workflow Note

Cat. No. 12927 · Fragment-enrichment workflow for selective recovery of shorter cfDNA fractions

Lysis and size-selection setup Magnetic fragment enrichment and washing Elution

Workflow — 5 mL Representative Plasma Input

Estimated Total Time≈ 110–140 min

Selection tuning

Selection Solution	Approximate Fragment Removal Target	Typical Use Logic
1000 μl	Remove fragments above ~1 kb	Broader retention of circulating DNA, less aggressive size cutoff
1250 μl	Remove fragments above ~600 bp	Intermediate enrichment for shorter cfDNA fractions
1500 μl	Remove fragments above ~500 bp	Stronger enrichment of shorter cfDNA fragments

These cutoff values are described in the protocol as performance references based on marker-fragment testing. Users can adjust the Selection Solution volume according to their own validation results.

How to read this note

1. Workflow structure
This workflow separates sample pretreatment from the shared downstream purification steps. It is intended as a practical companion to the product manual rather than a replacement for the official protocol. The downstream steps follow a magnetic bead-based enrichment workflow rather than a standard total-cfDNA extraction workflow. This page uses a representative 5 mL plasma workflow.

2. Time interpretation
Protocol times stated in the product manual are retained where applicable. Steps without explicit timing are estimated for an experienced operator. This workflow includes one lysis stage, one large-fragment removal stage and one short-fragment capture stage, followed by washing, drying and two-step elution.

3. Workflow characteristics
This system is designed for selective enrichment of shorter cfDNA fragments through size-dependent magnetic bead separation. MagBind Particles are first used to capture larger DNA fragments, which are discarded. The remaining supernatant is then processed with MagPure Particles G to enrich shorter circulating DNA fractions.

4. Practical considerations
For standard vacuum-based cfDNA column workflows, refer to HiPure Circulating DNA Kit (Cat. No. IVD3182). For standard large-volume magnetic cfDNA extraction without fragment-enrichment logic, refer to MagPure Circulating DNA Maxi Kit (Cat. No. IVD5435). For lower-volume magnetic cfDNA workflows, refer to MagPure Circulating DNA Mini Kit (Cat. No. IVD5432). For lower-input enrichment workflows, refer to MagPure Circulating DNA Rich Mini Kit (Cat. No. 12917).